two dimensional structural analysis and expression of a new staphylococcus aureus adhesin based fusion protein

Authors

jamshid faghri department of bacteriology and virology, faculty of medicine , isfahan university of medical sciences, isfahan, iran

delavar shahbazzadeh biotechnology research centre, pasteur institute of iran, tehran, iran

kamran pooshang bagheri biotechnology research centre, pasteur institute of iran, tehran, iran

sharareh moghim department of bacteriology and virology, faculty of medicine , isfahan university of medical sciences, isfahan, iran

abstract

objective(s) staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. antibiotic therapy for vancomycin resistant s. aureus (vrsa) can not promise the eradication of infections. since adhesion is the major route of infections, adhesin based vaccine could suppress s. aureus infections. fibronectin binding protein a (fnbpa) and clumping factor a (clfa) are major responsible adhesions involved in s. aureus infections, so they could be candidate vaccine molecules against an extensive range of infections. this project intended to express a new fusion protein construct and analysis of biological activity regarding binding activity. materials and methods pfnba- clfa construct was transformed to escherichia coli bl21 (de3). transformant e. coli were grown in lb broth and induced with iptg and cellular extracts were separated on sds–page. rt-pcr was performed to verify expression. binding activity of fusion protein was studied using human gingival fibroblast (hgf) cell line. d1-d3 protein from unpublished study was used as control. results the expected fusion protein fragment showed by sds-page. rt-pcr verified the existence of mrna relating to expressed fusion protein. binding activity of s. aureus decreased after treatment of hgf cells with fusion protein. conclusion in total,binding activity of fusion protein was approximately two fold lesser than d1-d3 protein. it is supposed that the fusion protein could not be attached to its ligand easily and would be more accessible to antigen presenting cells and consequentlyprotective antibodies will be produced. this project is pending for in vivo infection study in animal model.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Two Dimensional Structural Analysis and Expression of a New Staphylococcus aureus Adhesin Based Fusion Protein

Objective(s) Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA)...

full text

Two Dimensional Structural Analysis and Expression of a New Staphylococcus aureus Adhesin Based Fusion Protein

OBJECTIVES Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA) ar...

full text

Construction of a New Fusion Protein Vector Associated to Fibronectin Binding Protein A and Clumping Factor A Derived from Staphylococcus aureus NCTC8325

Objective(s) Staphylococcus aureus is a leading cause of many nosocomial and community acquired infections. According to many reports, antibiotic therapy can not guarantee the eradication of S. aureus infections. Thus designing an adhesin based vaccine could restrain the S. aureus infections. This study designed for construction of a new fusion protein vaccine against S. aureus infections base...

full text

LytU-SH3b fusion protein as a novel and efficient enzybiotic against methicillin-resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging infectious agent worldwide. The ever growing antibiotic resistance has made the researchers to look for new anti-staphylococcal agents. Autolysins are staphylococcal enzymes that lyse bacterial cell wall for cell division. Autolysins can be used as novel enzybiotics (enzymes have antibiotic effects) for staphylococcal ...

full text

Molecular Cloning, Expression and Peroxidase Conjugation of Staphylococcus aureus Protein A

Background: Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures.Objectives: Molecular cl...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۵، شماره ۲، صفحات ۷۲۵-۷۳۸

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023